C156 Diseases Caused by Gram-Negative Enteric Bacilli

General features and principles

Epidemiology

Structure and function

Pathogenesis

Bacterial GoalHost ObstacleBacterial Solution
ExtraintestinalattachmentFlow of urine, mucociliary escalatorMultiple adhesins (e.g., type 1, S, and F1C fimbriae; Ppili)
Nutrient acquisitionfor growthNutrient sequestration (e.g., iron via intracellular storageand extracellular scavenging via lactoferrin andtransferrin)Cellular lysis (e.g., hemolysin), multiple mechanisms forcompeting for iron (e.g., siderophores) and othernutrients
Initial avoidance ofhost bactericidalactivityComplement, phagocytic cells, antimicrobial peptidesCapsular polysaccharide, lipopolysaccharide
Dissemination (withinhost and betweenhosts)Intact tissue barriersIrritant tissue damage resulting in increased excretion(e.g., toxins such as hemolysin), invasion of brainendothelium
Late avoidance of hostbactericidal activityAcquired immunity (e.g., specific antibodies), treatmentwith antibioticsCell entry, acquisition of antimicrobial resistance

Infectious syndromes

Diagnosis

Treatment

Prevention

Escherichia coli infections

Commensal strains

Extraintestinal pathogenic strains

PathotypeEpidemiologyClinicalSyndromeaDefining Molecular TraitResponsible GeneticElementb
STEC/EHEC/ST-EAECFood, water, person-to- person; allages, industrialized countriesHemorrhagiccolitis, hemolytic-uremic syndromeShiga toxinLambda-like Stx1- or Stx2-encoding bacteriophage
ETECFood, water; young children in andtravelers to developing countriesTraveler’s diarrheaHeat-stable and labileenterotoxins, colonizationfactorsVirulence plasmid(s)
EPECPerson-to-person; young childrenand neonates in developingcountriesWatery diarrhea, persistent diarrheaLocalized adherence,attaching and ewacing lesionon intestinal epitheliumEPEC adherence factor plasmidpathogenicity island (locus forenterocyte ewacement [LEE])
EIECFood, water; children in andtravelers to developing countriesWatery diarrhea, occasionally dysenteryInvasion of colonic epithelialcells, intracellularmultiplication, cell- to-cellspreadMultiple genes containedprimarily in a large virulenceplasmid
EAEC?Food, water; children in and travelers to developing countries; all ages, industrialized countriesTraveler’s diarrhea,acute diarrhea,persistent diarrheaAggregative/diwuseadherence, virulence factorsregulated by AggRChromosomal or plasmid-associated adherence and toxingenes

Diagnosis

Treatment

Intestinal pathogenic strains

Pathotypes

Diagnosis

Treatment

Klebsiella infections

Alcoholism (2/3), DM

Infectious syndromes

Pneumonia

  • Hospital acquired pneumonia? m/c
  • Red currant jelly like sputum (necrotizing pneumonia)
  • CXR: bulging fissure (??? ??? ??? ?? ? minor fissure? ??), ??? ?? ??

UTI

Abdominal infection

  • Liver abscess
  • Metastatic spread to distant sites
    • Eyes: endophthalmitis
    • CNS: meningitis

Other infections

Bacteremia

Diagnosis

Treatment

  • ESBL: carbapenem
  • Quinolone
  • 3rd cepha

Proteus infections

Enterobacter and Cronobacter infections

Serratia infections

Citrobacter infections

Morganella and Providencia infections

Edwardsiella infections

Infections caused by miscellaneous genera

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